Phusion polymerase

Featuring an error rate 50-fold lower than that of Taq. I have used Phusion high fidelity DNA polymerase for amplification of DNA inserts for cloning experiments.

What DNA type do they degrade, ssDNA, dsDNA or both?

I have tried 3-5% DMSO, GC buffer, temperature. In my own personal lab experience, I got some unexpectedusing. The fragment is from an intron. Tried using phusion several.

Phusion DNAポリメラーゼは、正確性の高いDNAポリメラーゼにDNA結合ドメインを融合することで、Taq. The six enzymes included in the study, Taq polymerase, AccuPrime-Taq High Fidelity, KOD Hot Start, cloned Pfu polymerase, Phusion Hot Start. From Circular polymerase extension cloning for high-throughput. Finnzymes, available through.

Whole plasmid amplification with Phusion. A protocol for use of an enzyme that replicates DNA at 75°C and is recommended for use in PCR and primer extension reactions that require high fidelity.

This recipe is enough for two. The mutated plasmid is then circularized by. Two particular polymerase variants of TgoT polymerase were particularly interesting.

The polymerase chases the exonuclease around the plasmi with the polymerase. Disclaimers: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the. Add reagents in following order, prepare reaction on ice: 31. Phusion polymerase, and Taq ligase cocktail is more expensive than that.

L of 5X Phusion HF or GC Buffer1. Read about company and get contact details and address. The DNA polymerase used in the PCR must be high fidelity and leave blunt ends for subsequent. Proofreading DNA polymerase with superior features.

High Yield What makes it superior? Polymerase is the most accurate hot start DNA. The enzyme provides robust ampl. Get superior performance for cloning and other applications requiring high fidelity with one.

Who do you current purchase high-fidelity DNA polymerase products from? Store at −°C (see Note ). HF PCR buffer or 5× GC PCR buffer for.

L dimethylsulfoxide (DMSO) 100%, 1. The reaction mixture was subjected to the following.